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Fig. 2 | Animal Diseases

Fig. 2

From: Isolation of a feline-derived feline panleukopenia virus with an A300P substitution in the VP2 protein and confirmation of its pathogenicity in dogs

Fig. 2

Phylogenetic analysis, isolation, and identification of FPV in this study. A VP2 nucleotide sequence-based phylogenetic tree of 65 new sequences and reference sequences retrieved from GenBank. VP2 gene sequences from this study are marked in black “●” with a green background, and 91 VP2 gene sequences from NCBI are marked in yellow (from China) and purple (from Others) backgrounds. The isolates for this study are indicated by the names of the FPV-positive samples in bold red, and the vaccine strain is marked in bold blue. B Isolation and identification of six FPV isolates. CPEs were observed every 24 h post FPV-251 infection (hpi) in F81 cells (200×); FPV-251 isolate-specific IFA (green fluorescence) was performed on F81 cells at 96 hpi (200×); and FPV-251 virions were observed under a transmission electron microscope (Scale bar = 100 nm)

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Animal Diseases

ISSN: 2731-0442