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Fig. 1 | Animal Diseases

Fig. 1

From: Preparation and interaction mechanism analysis of single-chain fragment variables against phenylethanolamine A

Fig. 1

Construction and characterization of the initial scFv library. A Agarose gel (1%) electrophoresis identification of total RNA. B Agarose gel (1%) electrophoresis identification of VL and VH. Lane M: marker DL2000 bp; lane 1: VL; lane 2: VH. C Agarose gel (1%) electrophoresis identification of the assembled full-length scFv. Lane M: DL5000 bp marker. D Colony PCR of 18 clones from the initial library. Lane M: marker DL5000 bp; lanes 1–18: random colonies

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Animal Diseases

ISSN: 2731-0442