Fig. 3

Soluble expression and purification of scFv-32. A SDS‒PAGE results of scFv-32 expression at different IPTG concentrations. Lanes 1–7: 0, 0.05, 0.1, 0.2, 0.5, 0.8 and 1.0 mmol/L IPTG; Lane M: 10–180 kDa marker. B SDS–PAGE result of scFv-32 antibody purification. Lanes 1–2, lanes 3–4, lanes 5–6, lanes 7–8, lanes 9–10, lanes 11–12, and lanes 13–14: the eluate contained imidazole concentrations of 25, 50, 100, 200, 300, 400 and 500 mmol/L, respectively; Lane M: 10–180 kDa marker. C Western blot result of soluble scFv-32. Lane 1: 0 mmol/L IPTG; Lanes 2–6: 1 mmol/L IPTG; Lane M: 10–180 kDa marker