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Fig. 4 | Animal Diseases

Fig. 4

From: Preparation and interaction mechanism analysis of single-chain fragment variables against phenylethanolamine A

Fig. 4

Homology modeling and molecular docking analysis of scFv-32. A 3D conformation of the scFv-32 antibody. B Ramachandran plot of the scFv-32 model. C Molecular docking of scFv-32 and PEAA. D 2D views of the interaction between PEAA and key amino acid residues. E SDS‒PAGE results of the alanine scanning and scFv-32 mutant fractions. Lane M: 10–180 kDa; Lanes 1–3: TYR228, ASP229 and VAL178 were mutated to alanine; Lane 4: GLY180 was mutated to TRP. F Ic-ELISA results for alanine scanning and the scFv-32 mutant

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Animal Diseases

ISSN: 2731-0442