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Fig. 1 | Animal Diseases

Fig. 1

From: Two nanobody-based immunoassays to differentiate antibodies against genotype 1 and 2 porcine reproductive and respiratory syndrome virus

Fig. 1

Immunization of camels and construction of the VHH library. A SDS-PAGE analysis of the recombinant PRRSV-1-N protein. B SDS-PAGE analysis of the recombinant PRRSV-2-N protein. C Antigenic analysis of the recombinant PRRSV-1-N protein using the positive pig sera for PRRSV as the first antibody. D Antigenic analysis of the recombinant PRRSV-2-N protein using the positive pig sera for PRRSV as the first antibody. E Titers of antibodies against anti-PRRSV-1 and -2-N proteins in the immunized camel by indirect ELISA. F The VHH genes were amplified by the first round of PCR. G The VHH genes were amplified by the second round of PCR.M: Molecular weight markers; Lane 1: pET28a vector control; Lane 2: Induction with 0.1 mM IPTG; Lane 3: Inclusion body in precipitation after sonication; Lane 4: Soluble protein in supernatant after sonication; Lane 5: Purified PRRSV-1 or -2-N protein

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Animal Diseases

ISSN: 2731-0442