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Fig. 2 | Animal Diseases

Fig. 2

From: Two nanobody-based immunoassays to differentiate antibodies against genotype 1 and 2 porcine reproductive and respiratory syndrome virus

Fig. 2

Screening and identification of specific nanobodies from the VHH library. A Titers of antibodies against anti-PRRSV-1 and -2-N proteins in the immunized camel by indirect ELISA. B Amino acid sequence alignment of eight nanobodies against anti-PRRSV-1 and -2-N proteins. C Western blotting analysis of eight nanobody-HRP fusion proteins secreted into the medium of HEK293T cells. Anti-HA monoclonal antibody was used as the first antibody, and HRP labeled goat anti-mouse antibody as the second antibody. M: Molecular weight markers; Lane 1: g1-2-PRRSV-Nb3-HRP; Lane 2: g1-2-PRRSV-Nb13-HRP; Lane 3: g1-2-PRRSV-Nb136-HRP; Lane 4: g1-PRRSV-Nb90-HRP; Lane 5: g1-PRRSV-Nb136-HRP; Lane 6: g2-PRRSV-Nb5-HRP; Lane 7: g2-PRRSV-Nb14-HRP; Lane 8: g2-PRRSV-Nb27-HRP. D Analysis of the binding ability of eight nanobody-HRP fusion proteins against PRRSV-1 and -2-N proteins by ELISA. E Comparisons of positive pig sera for PRRSV blocking five nanobody HRP fusion proteins to react with PRRSV-1-N protein. F Positive pig sera for PRRSV blocking six nanobody HRP fusion proteins to bind to PRRSV-2-N protein

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Animal Diseases

ISSN: 2731-0442