Two nanobody-based immunoassays to differentiate antibodies against genotype 1 and 2 porcine reproductive and respiratory syndrome virus

Table 2 Optimized dilution of testing pig sera separate for the g1-2-cELISA and g1-cELISA

cELISA	Sample No	Sera type	Dilutions of the pig serum samples
cELISA	Sample No	Sera type	1:10	1:20	1:40	1:80	1:160	1:320
g1-2-cELISA	1	Positive	0.14	0.15	0.24	0.31	0.54	0.51
		Negative	1.09	1.06	1.08	1.09	1.05	1.11
		P/N	0.12	0.14	0.22	0.29	0.52	0.46
	2	Positive	0.28	0.28	0.41	0.50	0.68	0.82
		Negative	1.09	1.06	1.08	1.09	1.05	1.11
		P/N	0.25	0.27	0.38	0.46	0.64	0.74
	3	Positive	0.20	0.24	0.40	0.56	0.69	0.81
		Negative	1.09	1.06	1.08	1.09	1.05	1.11
		P/N	0.19	0.22	0.37	0.51	0.66	0.73
	4	Positive	0.07	0.09	0.14	0.18	0.25	0.36
		Negative	1.09	1.06	1.08	1.09	1.05	1.11
		P/N	0.07	0.09	0.13	0.16	0.24	0.33
g1-cELISA	5	Positive	0.19	0.20	0.37	0.54	0.80	0.88
		Negative	0.97	0.92	0.95	0.96	0.93	0.95
		P/N	0.20	0.22	0.39	0.56	0.86	0.92
	6	Positive	0.25	0.25	0.36	0.46	0.50	0.66
		Negative	0.97	0.92	0.95	0.96	0.93	0.95
		P/N	0.26	0.28	0.38	0.48	0.54	0.70
	7	Positive	0.30	0.29	0.36	0.54	0.69	0.98
		Negative	0.97	0.92	0.95	0.96	0.93	0.95
		P/N	0.31	0.32	0.38	0.57	0.74	1.04
	8	Positive	0.08	0.09	0.23	0.34	0.39	0.47
		Negative	0.97	0.92	0.95	0.96	0.93	0.95
		P/N	0.08	0.10	0.24	0.36	0.42	0.50