Table 3 Primer sequences for qRT‒PCR quantification
Gene name | Accession No | Primer pair | Primer sequences (5′–3′) | Length of PCR products |
|---|---|---|---|---|
GAPDH | NM_001289745.3 | GAPDH/F | GTGAAAGTCGGAGTCAACGG | 184 |
GAPDH/R | CGTTCTCAGCCTTGACAGTG | |||
IL-1β | NM_015297469.1 | IL-1β/F | GCATCAAGGGCTACAAGCTC | 134 |
IL-1β/R | GTCCAGGCGGTAGAAGATGA | |||
IL-4 | NM_001007079.2 | IL-4/F | CCAGCACTGCCACAAGAACC | 163 |
IL-4/R | AGCTAGTTGGTGGAAGAAGGTACG | |||
IL-6 | NM_204628.2 | IL-6/F | AGATGCTCGTCCGGAACAAC | 130 |
IL-6/R | AGGTAGGTCTGAAAGGCGAACA | |||
IFN-γ | NM_205149.2 | IFNγ/F | AAGCTCCCGATGAACGACTTG | 125 |
IFNγ/R | TTGCATCTCCTCTGAGACTGGC |