Fig. 2

Effects of ICA supplementation on microstructure of tibial bone tissues. a Representative images of Goldner’s trichrome staining (magnification, 10×). b Effects of ICA on cortical bone histomorphometry parameters. c Effects of ICA on trabecular bone histomorphometry parameters. CON, basal diet. LCD, low calcium diet. ICA, icariin. ICA1, low calcium diet supplied with 0.5 g/kg ICA. ICA2, low calcium diet supplied with 2.0 g/kg ICA. Ct. Ar, cortical area ratio. CW, cortical width. Tb. Ar, percent trabecular area. Tb. Th, trabecular thickness. Tb. Sp, trabecular separation. Tb. N, trabecular number. Data are presented as the mean ± SD (n = 6). Means in each column with different alphabetic letters are significantly (P < 0.05) different