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Fig. 1 | Animal Diseases

Fig. 1

From: Development of an indirect immunofluorescence assay for PCV3 antibody detection based on capsid protein

Fig. 1

Verification of PCV3 Cap protein expression in Sf9 cells. a Cytopathic effect in Sf9 insect cells transfected with recombinant bacmids. The left panel shows Sf9 cells, the middle panel shows Sf9 cells transfected with rvBac-Sc, and the right panel shows rvBac-Pc-transfected Sf9 cells. b Identification of recombinant Cap protein expression by western blot. Lane 1, cell lysate of Sf9 cells infected with rvBac-Pc. Lane 2, cell lysate of rvBac-Sc-infected Sf9 cells. Lane 3, supernatant of rvBac-Pc-infected Sf9 cells. Lane 4, supernatant of Sf9 cells infected with rvBac-Sc. Lane 5, cell lysate of mock-infected Sf9 cells. M, Marker. c Identification and localization of PCV3 Cap protein in infected Sf9 cells by IFA. rvBac-Sc, Sf9 cells infected with rvBac-Sc. rvBac-Pc, Sf9 cells infected with rvBac-Pc. Con, mock-infected Sf9 cells

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Animal Diseases

ISSN: 2731-0442