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Table 1 Fluorescent observations of different treatments

From: Development of an indirect immunofluorescence assay for PCV3 antibody detection based on capsid protein

Analysis

 

Specific fluorescence

Nonspecific fluorescence

Cell number (104/well)

1.0

++

2.0

++++

4.0

++++

8.0

++++

Serum dilution

1:10

++++

1:20

++++

1:40

+++

1:80

++

1:160

+

Cross-reactivity of pathogens

PCV3

++++

PCV2

PRV

CSFV

PRRSV

  1. PCV3 Porcine circovirus type 3, PCV2 Porcine circovirus type 3, PRV pseudorabies virus, CSFV classical swine fever virus, PRRSV porcine reproductive and respiratory syndrome virus. Results are classified as -, +, ++, +++, or ++++ according to the number of positive cells and the intensity of fluorescence