Development of a duplex real-time PCR method for the detection of influenza C and D viruses

Animal Diseases

Table 1 ICV and IDV detected by duplex real-time PCR assay using different primer concentrations

Primer concentration (nM)	IDV	ICV	Probe concentration (nM)	IDV	ICV
Primer concentration (nM)	Cq values	Cq values	Probe concentration (nM)	Cq values	Cq values
400	34.82	12.00	100	34.82	13.28
500	36.44	12.92	150	36.44	12.90
600	38.21	12.73	200	38.21	12.70
700	39.89	12.59	250	39.89	12.67
800	–	12.41	300	–	12.56
900	–	12.38	350	–	12.45
1000	–	12.49	400	–	12.49
1100	–	12.55	450	–	12.63

“-”: No signal detected, negative (−), ICV, influenza C virus; IDV, influenza D virus