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Table 2 Sensitivity test results of duplex real-time PCR assay

From: Development of a duplex real-time PCR method for the detection of influenza C and D viruses

Pathogen

Concentration

Repeat times

Positive number

Positive rate

ICV

1 × 102

15

15

100%

1 × 101

15

15

100%

1 × 100

15

13

87%

IDV

1 × 102

15

15

100%

1 × 101

15

15

100%

1 × 100

15

9

60%

  1. The cutoff line of positivity was automatically decided using a Roche LightCycler® 96 Instrument. ICV influenza C virus, IDV influenza D virus
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Animal Diseases

ISSN: 2731-0442