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Table 3 Specificity test results of duplex real-time PCR

From: Development of a duplex real-time PCR method for the detection of influenza C and D viruses

Positive samples

Singleplex conventional PCR detection and DNA sequencinga

Duplex real-time PCR detectionb

ICV

IDV

JEV

CSFV

IAV

TTsuV1

TTsuV2

PRRSV

PCV2

PCV3

ICV

+

–

–

–

–

–

–

–

–

–

+

IDV

–

+

–

–

–

–

–

–

–

–

+

JEV

–

–

+

–

–

–

–

–

–

–

–

CSFV

–

–

–

+

–

–

–

–

–

–

–

IAV

–

–

–

–

+

–

–

–

–

–

–

TTsuV1

–

–

–

–

–

+

–

–

–

–

–

TTsuV2

–

–

–

–

–

–

+

–

–

–

–

PRRSV

–

–

–

–

–

–

–

+

–

–

–

PCV2

–

–

–

–

–

–

–

–

+

–

–

PCV3

–

–

–

–

–

–

–

–

–

+

–

  1. a These samples were detected by conventional PCR and confirmed by DNA sequencing. b Criteria of positivity: Cq ≤ 38, invalid (?); Cq > 38 or no signal detected, negative (−). PCV2 porcine circovirus type 2, PCV3 porcine circovirus type 3, JEV Japanese encephalitis virus, CSFV classical swine fever virus; IAV influenza A virus, TTsuV1 Torque teno sus virus 1, TTsuV2 Torque teno sus virus 2, PRRSV porcine reproductive and respiratory syndrome virus, ICV influenza C virus, IDV influenza D virus