From: Development of a duplex real-time PCR method for the detection of influenza C and D viruses
Plasmid | Concentration | Intra-assay Cq value | Interassay Cq value | ||
---|---|---|---|---|---|
Mean ± SDa | CV% | Mean ± SD | CV% | ||
ICV | 1 × 107 | 13.26 ± 0.26 | 1.96 | 13.79 ± 0.26 | 1.91 |
1 × 106 | 16.65 ± 0.16 | 0.96 | 16.86 ± 0.22 | 1.33 | |
1 × 105 | 20.41 ± 0.06 | 0.29 | 20.54 ± 0.13 | 0.65 | |
1 × 104 | 24.45 ± 0.08 | 0.33 | 24.03 ± 0.24 | 0.99 | |
1 × 103 | 27.51 ± 0.02 | 0.07 | 27.55 ± 0.29 | 1.06 | |
1 × 102 | 31.16 ± 0.1 | 0.32 | 31.86 ± 0.21 | 0.67 | |
1 × 101 | 34.51 ± 0.06 | 0.17 | 34.51 ± 0.39 | 1.12 | |
IDV | 1 × 107 | 15.03 ± 0.1 | 0.67 | 15.42 ± 0.25 | 1.63 |
1 × 106 | 18.23 ± 0.03 | 0.16 | 18.93 ± 0.27 | 1.43 | |
1 × 105 | 21.73 ± 0.18 | 0.83 | 21.74 ± 0.15 | 0.68 | |
1 × 104 | 25.03 ± 0.25 | 1 | 26.02 ± 0.22 | 0.84 | |
1 × 103 | 28.62 ± 0.32 | 1.11 | 29.26 ± 0.26 | 0.87 | |
1 × 102 | 32.03 ± 0.05 | 0.16 | 32.14 ± 0.21 | 0.66 | |
1 × 101 | 32.89 ± 0.21 | 0.64 | 34.74 ± 0.53 | 1.52 |