Development of a duplex real-time PCR method for the detection of influenza C and D viruses

Animal Diseases

Table 5 Primer and probe sequences for ICV and IDV

Primer/Probe	Sequence 5′-3′	Gene	Product size	Position
ICV-Detect-F	AAGAGCCATGCAAGGAATTGA	NP	117 bp	710-826^a
ICV-Detect-R	TCTGCGATGACATTTAGAGCTTC
ICV-Probe	VIC-AAAGCGTTTCCTGGACCTTTAA-MGB
ICV-RT-F	ATGGGRCGAATGGCDATGAAATGGT	M	257 bp	731-987^a
ICV-RT-R	TCCCATCTGCCRAGYACAAAACC
IDV-Detect-F	CCCTCCTACAGGAACAGAGTTTTC	PB1	124 bp	1880-2003^b
IDV-Detect-R	GCGTTGATACAACTGCCTCG
IDV-Probe	FAM-AGGAACCCCTTTACACAGTTTGAGAAGACAG-BHQ1
IDV-RT-F	GTGGTGCACATATGCCTGAG	P42	487 bp	169-655^b
IDV-RT-R	CCATTGCTCTTGAYCCATAYGC