Table 8 Primers of antimicrobial resistance genes and virulence genes
Classification | Gene | Primer sequence (5′ → 3′) | Annealing temperature | Fragment length | Reference |
|---|---|---|---|---|---|
β-lactams | blaOXA | F:TTTTCTGTTGTTTGGGTTTC R:TTTCTTGGCTTTTATGCTTG | 53 °C | 447 bp | This work |
blaSHV | F:TGTATTATCTCCCTGTTAGC R:TTAGCGTTGCCAGTGCTC | 55 °C | 843 bp | ||
blaTEM | F:CAGAAACGCTGGTGAAAG R:TTACCAATGGTTAATCAGTGAG | 54 °C | 788 bp | ||
Tetracyclines | tet (A) | F:GCTACATCCTGCTTGCCTTC R:CATAGATCGCCGTGAAGAGG | 59.5 °C | 210 bp | Ng et al. 2001 |
tet (B) | F:TTGGTTAGGGGCAAGTTTTG R:GTAATGGGCCAATAACACCG | 59.5 °C | 659 bp | ||
tet (C) | F:CTTGAGAGCCTTCAACCCAG R:ATGGTCGTCATCTACCTGCC | 59.5 °C | 418 bp | ||
Sulfonamides | sul1 | F:TCGGACAGGGCGTCTAAG R:GGGTATCGGAGCGTTTGC | 63 °C | 925 bp | This work |
sul2 | F:CTTGTTTCGTCCGACACAGA R:GAAGCGCAGCCGCAATTCAT | 60 °C | 435 bp | ||
Aminoglycosides | aadA1 | F:GCAGCGCAATGACATTCTTG R:ATCCTCGGCGCGATTTTG | 60 °C | 282 bp | Sáenz et al. 2004 |
aadB | F:GAGGAGTTGGACTATGGATT R:CTTCATCGGCATAGTAAAA | 53 °C | 208 bp | This work | |
aac (3′)-IIa | F:GGCGACTTCACCGTTTCT R: GGACCGATCACCCTACGAG | 54 °C | 412 bp | ||
Chloramphenicols | floR | F: GAACACGACGCCCGCTAT R: TTCCGCTTGGCCTATGAG | 54 °C | 601 bp | This work |
Yersinia High Pathogenicity Island | irp2 | F:AAGGATTCGCTGTTACCGGA R:TCGGCCAGGATGATTCGTCG | 60 °C | 301 bp | This work |
fyuA | F:ACACGGCTTATCCTCTGGC R:GGCATCTTGACGATTAACGAA | 58 °C | 953 bp | This work | |
Intimin | eaeA | F:ATTACTGAGATTAAGGCTGAT R:ATTTATTTGCAGCCCCCCAT | 57 °C | 682 bp | This work |
Fimbriae | F41 | F:GAGGGACTTTCATCTTTTAG  R:AGTCCATTCCATTTATAGGC | 58 °C | 431 bp | This work |
K88 | F:GCTGCATCTGCTGCATCTGGTATG  R:CCACTGAGTGCTGGTAGTTACAGCC | 60 °C | 792 bp | This work | |
K99 | F:TATTATCTTAGGTGGTATGG  R:GGTATCCTTTAGCAGCAGTATTTC | 56 °C | 314 bp | This work | |
987P | F:TCTGCTCTTAAAGCTACTGG  R:AACTCCACCGTTTGTATCAG | 55.8 °C | 333 bp | This work | |
F18 | F:GTGAAAAGACTAGTGTTTATTTC R:CTTGTAAGTAACCGCGTAAGC | 55 °C | 510 bp | This work | |
Hemolysin | hylA | F:GCATCATCAAGCGTACGTTCC R:AATGAGCCAAGCTGGTTAAGCT | 60 °C | 534 bp | This work |
Shiga toxins | Stx1 | F:TTAGACTTCTCGACTGCAAAG R:TGTTGTACGAAATCCCCTCTG | 52 °C | 531 bp | This work |
Stx2 | F:CCATGACAACGGACAGCAGTT R:CCTGTCAACTGAGCAGCACTTTG | 58 °C | 779 bp | This work | |
Heat-stable enterotoxins | STa | F:TCCCCTCTTTTAGTCAGTCAACTG R:GCACAGGCAGGATTACAACAAAGT | 56 °C | 163 bp | This work |
STb | F:GCAATAAGGTTGAGGTGAT R:GCCTGCAGTGAGAAATGGAC | 60 °C | 368 bp | This work | |
Heat-labile enterotoxins | LT | F:GGCGACAGATTATACCGTGC R:CGGTCTCTATATTCCCTGTT | 54 °C | 450 bp | This work |