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Fig. 1 | Animal Diseases

Fig. 1

From: Inactivated Pseudomonas PE(ΔIII) exotoxin fused to neutralizing epitopes of PEDV S proteins produces a specific immune response in mice

Fig. 1

Schematic and expression of the recombinant protein. a PE, full-length sequence of PE toxin contains four domains (Ia, II, Ib, III); PE (ΔIII) represents full-length sequence of a PE toxin deletant that deleted domain III. S-A, PEDV S1 aa 25-229; S-B, PEDV S aa 499-789+aa 1370-1378. b SDS-PAGE analysis of PE(ΔIII)-PEDV-S-A and PE(ΔIII)-PEDV-S-B proteins purified through affinity chromatography. Lane 1: PE(ΔIII)-PEDV-S-A (71 kDa). Lane 2: PE(ΔIII)-PEDV-S-B (80.5 kDa). c Western blot analysis of PE(III)-PEDV-S-A and PE(III)-PEDV-S-B proteins. Lane 1: PE(ΔIII)-PEDV-S-A. Lane 2: PE(ΔIII)-PEDV-S-B. Mouse anti-HisMAb and goat anti-mouse IgG-HRP were used as primary and secondary antibodies, respectively. PE, Pseudomonas aeruginosa exotoxin A; PEDV, porcine epidemic diarrhea virus

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Animal Diseases

ISSN: 2731-0442