Fig. 1

PEDV nsp14 inhibites NF-κB promoter activation. A HEK-293 T cells were transfected with pNF-κB-Luc reporter plasmid (0.05 μg) and pRL-TK plasmid (0.005 μg) together with empty vector plasmids or plasmids expressing PEDV nsp10 or nsp14 (0.1 μg) for 24 h. Cells were then mock-infected or infected with SeV for another 16 h, and luciferase activities were measured. Expression of nsp10 and nsp14 was analyzed by Western blotting using an anti-Flag antibody. B HEK-293 T cells were transiently transfected with pNF-κB-Luc reporter plasmid (0.05 μg) and pRL-TK plasmid (0.005 μg) together with increasing amounts of plasmids expressing PEDV nsp14 (0, 0.01, 0.05, 0.1 or 0.2 μg). At 24 hpt, cells were mock-infected or infected with SeV for another 16 h. Luciferase activities were measured using a dual luciferase reporter assay. Expression of nsp14 was analyzed via Western blotting with an anti-Flag antibody. Data are expressed as the means ± SD from three independent experiments. *, P < 0.05; **, P < 0.01. ns indicates not significant. C IPEC-J2 cells were transiently transfected with pNF-κB-Luc reporter plasmid and pRL-TK plasmid together with empty vector plasmids or plasmids expressing PEDV nsp14 for 24 h. Cells were then mock-infected or infected with SeV for another 24 h, and luciferase activities were measured