Fig. 4From: Porcine epidemic diarrhea virus nsp14 inhibits NF-κB pathway activation by targeting the IKK complex and p65PEDV nsp14 did not inhibit protein expression of various components of NF-κB pathway. HEK293T cells were transfected with constructs expressing HA-tagged RIG-I, VISA, IKKα, IKKβ, IκBα or p65 along with vector plasmids or Flag-nsp10 expression plasmids. At 24 hpt, cell lysates were prepared for Western blotting analysis with the indicated antibodies. The relative abundance of the detected proteins was determined by densitometric analysis using ImageJ softwareBack to article page