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Fig. 5 | Animal Diseases

Fig. 5

From: Porcine epidemic diarrhea virus nsp14 inhibits NF-κB pathway activation by targeting the IKK complex and p65

Fig. 5

PEDV nsp14 interactes with IKKα, IKKβ and p65 in NF-κB pathway. A and B HEK-293 T cells were cotransfected with Flag-nsp14 expression plasmids and the indicated HA-tagged molecule expression plasmids (RIG-I, VISA, IKKα, IKKβ, IκBα or p65). At 36 hpt, cell lysates were prepared for Co-IP experiments using anti-Flag antibody (A), anti-HA antibody or (B) nonspecific mouse IgG. Western blotting analysis was carried out with the indicated antibodies to evaluate the whole cell lysates (WCLs) and immunoprecipitation complexes. C IPEC-J2 cells were transfected with Flag-nsp14 expression plasmids for 36 h. Then, cell lysates were prepared for Co-IP experiments using anti-Flag antibody or nonspecific mouse IgG. Western blotting analysis was carried out with the indicated antibodies to evaluate WCLs and IP complexes

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Animal Diseases

ISSN: 2731-0442