Fig. 6

PEDV nsp14 inhibites IKKα/β and p65 phosphorylation and nuclear translocation of NF-κB. A HEK293T cells were transfected with 1 μg vector plasmids or Flag-nsp14 expression plasmids. At 24 hpt, cells were treated with TNF-α (30 ng/mL) or mock-treated for 30 min. Cell lysates were then prepared, and the target proteins were detected by Western blotting. B Double immunostained of transfected HEK293T cells treated in the same way as with anti-Flag (red) and anti-p65 (green) antibodies