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Fig. 1 | Animal Diseases

Fig. 1

From: Rationally designed mariner vectors for functional genomic analysis of Actinobacillus pleuropneumoniae and other Pasteurellaceae species by transposon-directed insertion-site sequencing (TraDIS)

Fig. 1

General map of mariner vectors, pTsodCPC9 and pTlacPC9, differing only in the promoter for the tnp gene. Genes tnp (mariner transposase C9 mutant), cat (chloramphenicol resistance), traJ (plasmid transfer gene), and bla (ampicillin resistance) are indicated by the appropriately labelled solid red arrows. The origin of plasmid transfer (oriT) is shown as a filled lollipop (), and the origin of plasmid replication (colE1) as a hollow lollipop (). Coloured blocks flanking the cat gene indicate the locations of Himar1 inverted repeats (thick orange), and paired copies of DNA uptake sequences for Neisseria spp. (thin green), H. influenzae (thin pink) and A. pleuropneumoniae (thin blue). Arrowhead upstream of the C9 tnp gene indicates promoter sequences for either A. pleuropneumoniae sodC or E. coli lac genes, depending on the vector (pTsodCPC9 and pTlacPC9, respectively)

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