Rationally designed mariner vectors for functional genomic analysis of Actinobacillus pleuropneumoniae and other Pasteurellaceae species by transposon-directed insertion-site sequencing (TraDIS)

Table 2 Location of mariner insertions in anaerobic mutants of P. multocida

Gene	Product of disrupted gene	Gene location^a	Location of insert^a
MIDG3277_1258	Predicted glycosyl transferase	contig21:104818..105990^b	contig21:105340
aroG	Deoxy-7-phosphoheptulonate synthase	contig15:68162..69247	contig15:68149^c
intergenic	Periplasmic pH-dependent serine endoprotease DegQ	contig 16:46775..48154	contig16:46700^d
MIDG3277_1016	Hypothetical protein^e	contig18:76828..77169C	contig18:77129
aroA	3-phosphoshikimate 1-carboxyvinyltransferase	contig18:79640..80962	contig18:80029
aroA	3-phosphoshikimate 1-carboxyvinyltransferase	contig18:79640..80962	contig18:80287
sucC	Succinate--CoA ligase subunit beta	contig3:86080..87246	contig3:86908
ccmD	Heme exporter protein CcmD	contig1:16084..16287	contig1:16136
MIDG3277_1814	F₀F₁ ATP synthase subunit I	contig31:36319..36726	contig31:36262^f
atpG	F₀F₁ATP synthase subunit gamma	contig31:40520..41389	contig31:40497^g
MIDG3277_0379	Hypothetical protein	contig6:168..512^h	contig6:434
pfeA	Ferric enterobactin receptor	contig27:62820..64826C	contig27:64243
menA	1,4-dihydroxy-2-naphthoate octaprenyltransferase	contig23:97896..98801C	contig23:98875
menB	1,4-dihydroxy-2-naphthoyl-CoA synthase	contig23:34911..35768C	contig23:35383
menC	O-succinylbenzoate synthase	contig23:33645..34658C	contig23:34159

^aLocation in the draft genome of MIDG3277 (accession number ERZ681052)
^bStart and end positions of genes are indicated; those on the complementary strand are shown with the suffix C
^cInsertion located 13 bases upstream of the start of aroG; insertion likely disrupts transcription of aroG
^dInsertion located 75 bases upstream of the start of degQ and 147 bases upstream of the start of MIDG3277_0890 encoding a putative NAD(P)H nitroreductase; insertion likely disrupts transcription of degQ
^eThis protein shares 100% identity with PM0836 (Genbank accession AAK02920) which was reported to be in vivo expressed (Hunt et al. 2001)
^fInsertion located 57 bases upstream of the start of atpI (MIDG3277_1814); insertion likely disrupts transcription of atpI and remaining genes in the atp operon
^gInsertion located 23 bases upstream of atpG; insertion likely disrupts transcription of atpG and remaining genes in the atp operon
^hThis predicted CDS is on a small contig and may be part of a larger gene (100% identity with the last 104 AAs of a 818 AA hypothetical protein P1062_0208970; Genbank accession ESQ71762)