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Table 3 Primers used in this study

From: Rationally designed mariner vectors for functional genomic analysis of Actinobacillus pleuropneumoniae and other Pasteurellaceae species by transposon-directed insertion-site sequencing (TraDIS)

Name

Sequence

CmDUSUSS_for

CGCGGATGCCGTCTGAAGTGCGGTACAAGCGGTCGGCAATAGTTACC

CmDUSUSS_rev

CGCGAAGTGCGGTATGCCGTCTGAACAAGCGGTTTCAACTAACGGG

CmDUSUSS_IRleft

CTGATAAGTCCCCGGTCTGCAGGCGGCCGCACTAGTGATTC

CmDUSUSS_IRright

CTGATAAGTCCCCGGTCTCGAAGTGCGGTATGCCGTCTG

Himar_IR

TAACAGGTTGGCTGATAAGTCCCCGGTCT

ISceI_left

TAGGGATAACAGGGTAATCATGGCCGCGGGATTAACAGGTTG

ISceI_right

ATTACCCTGTTATCCCTACGGCCGCACTAGTGATTAACAGG

M13_for

GTAAAACGACGGCCAGTG

M13_rev

GGAAACAGCTATGACCATG

oriTtraJ_left

CCGCCTGCAGGTCGACAAAACAGCAGGGAAGCAGCGCTTTTC

oriTtraJ_right

ACTCAAGCTATGCATGCATGGGGACGTGCTTGGCAATC

sodCPC9_left

CGAATTGGGCCCGACCGCCAACCGATAAAACCTACATTTTGC

sodCPC9_right

CCTCCTTTTCTAGTCGCGGTACCGTCGACTGCAGAATTC

lacPC9_left

CGAATTGGGCCCGACGTGAGCGCAACGCAATTAATGTGAGTTAG

lacPC9_right

CCTCCTTTTCTAGTCGGCGTAATCATGGTCATAGCTGTTTCC

C9sodC_left

AGTCGACGGTACCGCGACTAGAAAAGGAGGATTCCTCATATGG

C9lacP_left

GACCATGATTACGCCGACTAGAAAAGGAGGATTCCTCATATGG

C9_right

CCGGGAGCATGCGACCCAGTGTGCTGGAATTCGCCCTTAGC

L-PCR-C

GATAAGCAGGGATCGGAACC

IR-Left_out

CACTTCAGACGGCATCCGCGAATC

IR-Right_out

GTTGAAACCGCTTGTTCAGACGGC

  1. Specific DNA uptake sequences for Neisseria spp. (underlined), H. influenzae (italics), and A. pleuropneumoniae (bold) are indicated in the CmDUSUSS_for and CmDUSUSS_rev sequences
  2. The ISceI restriction site is indicated in bold italic text
  3. The 15 base overhangs for In Fusion cloning are shown in subscript text on the 5′ end of primers oriTtraJ_left to C9_right