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Table 1 Strains, plasmids and protein used in this study

From: The QseB/QseC two-component system contributes to virulence of Actinobacillus pleuropneumoniae by downregulating apf gene cluster transcription

Strains or plasmids Characteristics
A. pleuropneumoniae
 S4074 A. pleuropneumoniae reference strain of serovar 1; WT strain
 ΔqseBC A. pleuropneumoniae S4074 qseBC-deletion mutant
 ΔapfA A. pleuropneumoniae S4074 apfA-deletion mutant
 ΔapfB A. pleuropneumoniae S4074 apfB-deletion mutant
 ΔapfC A. pleuropneumoniae S4074 apfC-deletion mutant
 ΔapfD A. pleuropneumoniae S4074 apfD-deletion mutant
 CΔqseBC Complemented strain of ΔqseBC; Cmr
 CΔapfA Complemented strain of ΔapfA; Cmr
 CΔapfB Complemented strain of ΔapfB; Cmr
 CΔapfC Complemented strain of ΔapfC; Cmr
 CΔapfD Complemented strain of ΔapfD; Cmr
E. coli
 DH5α Cloning host for recombinant vector
 β2155 Transconjugation donor for constructing mutant strain
 BL21 (DE3) The expression host for pET-30a and their derivative
Plasmids
 pEMOC2 Transconjugation vector: ColE1 ori mob RP4 sacB, Ampr Cmr
 pEΔqseBC Up- and down-stream arms of qseBC were ligated sequentially into pEMOC2, and used as the transconjugation vector for qseBC gene deletion
 pEΔapfA Up- and down-stream arms of apfA were ligated sequentially into pEMOC2, and used as the transconjugation vector for apfA gene deletion
 pEΔapfB Up- and down-stream arms of apfB were ligated sequentially into pEMOC2, and used as the transconjugation vector for apfB gene deletion
 pEΔapfC Up- and down-stream arms of apfC were ligated sequentially into pEMOC2, and used as the transconjugation vector for apfC gene deletion
 pEΔapfD Up- and down-stream arms of apfD were ligated sequentially into pEMOC2, and used as the transconjugation vector for apfD gene deletion
 pJFF224-XN E. coli-APP shuttle vector: RSF1010 replicon; mob oriV, Cmr
 pJFF-qseBC pJFF224-XN carrying the intact qseBC
 pJFF-apfA pJFF224-XN carrying the intact apfA
 pJFF-apfB pJFF224-XN carrying the intact apfB
 pJFF-apfC pJFF224-XN carrying the intact apfC
 pJFF-apfD pJFF224-XN carrying the intact apfD
 pET-30a Expression vector; Kanr
 pET-qseB pET-30a carrying qseB gene
  1. Cmr, chloramphenicol resistance, Ampr, ampicillin resistance, Kanr, kanamycin resistance