The QseB/QseC two-component system contributes to virulence of Actinobacillus pleuropneumoniae by downregulating apf gene cluster transcription

Duan, Benzhen; Peng, Wei; Yan, Kang; Liu, Feng; Tang, Jia; Yang, Fengming; Chen, Huanchun; Yuan, Fangyan; Bei, Weicheng

doi:10.1186/s44149-022-00036-w

Table 1 Strains, plasmids and protein used in this study

From: The QseB/QseC two-component system contributes to virulence of Actinobacillus pleuropneumoniae by downregulating apf gene cluster transcription

Strains or plasmids	Characteristics
A. pleuropneumoniae
S4074	A. pleuropneumoniae reference strain of serovar 1; WT strain
ΔqseBC	A. pleuropneumoniae S4074 qseBC-deletion mutant
ΔapfA	A. pleuropneumoniae S4074 apfA-deletion mutant
ΔapfB	A. pleuropneumoniae S4074 apfB-deletion mutant
ΔapfC	A. pleuropneumoniae S4074 apfC-deletion mutant
ΔapfD	A. pleuropneumoniae S4074 apfD-deletion mutant
CΔqseBC	Complemented strain of ΔqseBC; Cm^r
CΔapfA	Complemented strain of ΔapfA; Cm^r
CΔapfB	Complemented strain of ΔapfB; Cm^r
CΔapfC	Complemented strain of ΔapfC; Cm^r
CΔapfD	Complemented strain of ΔapfD; Cm^r
E. coli
DH5α	Cloning host for recombinant vector
β2155	Transconjugation donor for constructing mutant strain
BL21 (DE3)	The expression host for pET-30a and their derivative
Plasmids
pEMOC2	Transconjugation vector: ColE1 ori mob RP4 sacB, Amp^r Cm^r
pEΔqseBC	Up- and down-stream arms of qseBC were ligated sequentially into pEMOC2, and used as the transconjugation vector for qseBC gene deletion
pEΔapfA	Up- and down-stream arms of apfA were ligated sequentially into pEMOC2, and used as the transconjugation vector for apfA gene deletion
pEΔapfB	Up- and down-stream arms of apfB were ligated sequentially into pEMOC2, and used as the transconjugation vector for apfB gene deletion
pEΔapfC	Up- and down-stream arms of apfC were ligated sequentially into pEMOC2, and used as the transconjugation vector for apfC gene deletion
pEΔapfD	Up- and down-stream arms of apfD were ligated sequentially into pEMOC2, and used as the transconjugation vector for apfD gene deletion
pJFF224-XN	E. coli-APP shuttle vector: RSF1010 replicon; mob oriV, Cm^r
pJFF-qseBC	pJFF224-XN carrying the intact qseBC
pJFF-apfA	pJFF224-XN carrying the intact apfA
pJFF-apfB	pJFF224-XN carrying the intact apfB
pJFF-apfC	pJFF224-XN carrying the intact apfC
pJFF-apfD	pJFF224-XN carrying the intact apfD
pET-30a	Expression vector; Kan^r
pET-qseB	pET-30a carrying qseB gene

Cm^r, chloramphenicol resistance, Amp^r, ampicillin resistance, Kan^r, kanamycin resistance

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Animal Diseases