Primers | Sequence (5′-3′)a |
---|---|
For mutant construction | |
 qseBC-up-F/R | TTGTCGACCTATGGCTTTAACTTCCTTCG |
GGGAATTCATTATTGTCCGATAAAGCGAC | |
 qseBC-down-F/R | GGGAATTCTTAAGCCGAAGAGAAAAAGACG |
GGTCTAGAGTCGTATCGTTTAATGCGATC | |
 apfA-up-F/R | CTGTCGACTTTGATACCGAGCTGCAATAAGCGT |
GCGTTTACTTTAATTGAATTGATGAGGAAACGAAATGTAAAGGGGAGGAC | |
 apfA-down-F/R | GTCCTCCCCTTTACATTTCGTTTCCTCATCAATTCAATTAAAGTAAACGC |
ATGCGGCCGCCGCTACGGCTGAGGAAAATC | |
 apfB-up-F/R | CTGTCGACCGATAATCAGCATTAAGATCGGCTC |
GAAATACCTGCTCGGGCAATTAATATTGTTATCACGGCTACAGAGGC | |
 apfB-down-F/R | GCCTCTGTAGCCGTGATAACAATATTAATTGCCCGAGCAGGTATTTC |
ATGCGGCCGCTACATTCTTTGCATTGCCCCAT | |
 apfC-up-F/R | CTGTCGACGGCTTGTATTTGGCTTAACATCTCT |
ACAACTTCAGAAAGGAAAAGCGTATTATCGGTACGGTTTTGATCGGAT | |
 apfC-down-F/R | ATCCGATCAAAACCGTACCGATAATACGCTTTTCCTTTCTGAAGTTGT |
ATGCGGCCGCCGAGATTTTTGCTTTTCTTACGC | |
 apfD-up-F/R | CTGTCGACCATTTGCTTTCCTTATCTTTTGCTA |
GTGGTGTAAGCGAAGTATTCCTCAATTTTCGACTTTCGTGCTAATTATTG | |
 apfD-down-F/R | CAATAATTAGCACGAAAGTCGAAAATTGAGGAATACTTCGCTTACACCAC |
ATGCGGCCGCGCTGAATGATTTAAACCGTTTGATA | |
 apxIVA-F/R | GGCTACCCATTTCCCTTCG |
GAGCAACAACGTCGCACA | |
 qseBC-exterior-F/R | GTTTATCGGTAGGTCCACAGG |
TGATCGCAGCAAATACCAAA | |
 qseBC-interior-F/R | CGGAACGACTCGCCTCTT |
AAATCGCCAAAATCAATAGCGGTAG | |
 apfA-exterior-F/R | AATACCGGCTCAATTCGCTTA |
ACCAGTCACGTAAAGGGTCGGC | |
 apfA-interior-F/R | GGTGTCATCGTATAACCGTATT |
TTGGCGGCATCGGCTTCTT | |
 apfB-exterior-F/R | GTTGGATACGGATCTGTGAATAG |
TTACGGTGGAAGGCGGAACGATTAC | |
 apfB-interior-F/R | CGCATCATTGGTATGTAAGGTC |
GGATTTCCGTGTTTCGAGTTT | |
 apfC-exterior-F/R | AGGAATACTTCGCTTACACCAC |
GTTATCACGGCTACAGAGGC | |
 apfC-interior-F/R | CTAATCGCCCGCTTTGTT |
CTGAATGCCGCAGTCCCT | |
 apfD-exterior-F/R | CATCATCGGCATAACGCAGTC |
AGGCGGATTAGTCGGAGGC | |
 apfD-interior-F/R | GCATAAACCCAGCATACCA |
TTCCTCTGCTTGCCTTTG | |
For complement construction | |
 qseBC-F/R | AACTGCAGTTGCAAAATTTCAGCAAATTTAGAC |
TTGCGGCCGCTTAGAAGATAATTTCCGCTTTTAAA | |
 apfA-F/R | CCCTCGAGATGCAAAAACTAAGTCTTATTCGAC |
TTGCGGCCGCTTAATTTGATGCGCAGAAATTTGCC | |
 apfB-F/R | CCCTCGAGATGCAGTATTCCGTATGTGATGTAA |
TTGCGGCCGCTCATACACTTTCAACATGACCTAAC | |
 apfC-F/R | CCCTCGAGATGTTGAAAGTGTATGAATTTCATT |
TTGCGGCCGCCTAACCGCCTCCGACTAATCCGCCT | |
 apfD-F/R | CCCTCGAGATGAGTTCTCCGATTTTCGCATGGT |
TTGCGGCCGCTTAGATATAAATTTTATCAATAATT | |
For RT-qPCR | |
 16S rRNA-F/R | CCATGCCGCGTGAATGA |
TTCCTCGCTACCGAAAGAACTT | |
 apfA-qPCR-F/R | GACCGCCGCTACAGTTTTTA |
TTGCCATTTTAGCTACGGTTG | |
 apfB-qPCR-F/R | CGAAGGCGCATCATTGGTAT |
CAAACGCAAATAAACCCGGC | |
 apfC-qPCR-F/R | AAATTTCGCCTAATCGCCCG |
GCCGCAGTCCCTTTAAAACA | |
 apfD-qPCR-F/R | TGTCCCCGCTTCCCAATATT |
TGCTTGCCTTTGGAATTGCT | |
For protein expression | |
 PqseB-F/R | CGCCCATATGTTGCAAAATTTCAGCAAATTTAGAC |
CGCCCTCGAGAGCATCTGAATTTCCTATTTTA | |
For EMSAs | |
 apf-EMSA-F/R | ATCCCTCTCCTTATCATATCTAAGA |
TTTGACCTCTGAATTTATTAAAACG | |
 pilM-EMSA-F/R | CGAAAAATTCTCTTCTGGTAAATGA |
AATTTTAGTTCCTTAAATCAACTTT | |
 glpK-EMSA-F/R | CGTTTCCCTTTTTTACGGACTAAAC |
ATAGTCCTCCTACGGATAACGAAAA |