For mutant construction
|
qseBC-up-F/R
|
TTGTCGACCTATGGCTTTAACTTCCTTCG
|
GGGAATTCATTATTGTCCGATAAAGCGAC
|
qseBC-down-F/R
|
GGGAATTCTTAAGCCGAAGAGAAAAAGACG
|
GGTCTAGAGTCGTATCGTTTAATGCGATC
|
apfA-up-F/R
|
CTGTCGACTTTGATACCGAGCTGCAATAAGCGT
|
GCGTTTACTTTAATTGAATTGATGAGGAAACGAAATGTAAAGGGGAGGAC
|
apfA-down-F/R
|
GTCCTCCCCTTTACATTTCGTTTCCTCATCAATTCAATTAAAGTAAACGC
|
ATGCGGCCGCCGCTACGGCTGAGGAAAATC
|
apfB-up-F/R
|
CTGTCGACCGATAATCAGCATTAAGATCGGCTC
|
GAAATACCTGCTCGGGCAATTAATATTGTTATCACGGCTACAGAGGC
|
apfB-down-F/R
|
GCCTCTGTAGCCGTGATAACAATATTAATTGCCCGAGCAGGTATTTC
|
ATGCGGCCGCTACATTCTTTGCATTGCCCCAT
|
apfC-up-F/R
|
CTGTCGACGGCTTGTATTTGGCTTAACATCTCT
|
ACAACTTCAGAAAGGAAAAGCGTATTATCGGTACGGTTTTGATCGGAT
|
apfC-down-F/R
|
ATCCGATCAAAACCGTACCGATAATACGCTTTTCCTTTCTGAAGTTGT
|
ATGCGGCCGCCGAGATTTTTGCTTTTCTTACGC
|
apfD-up-F/R
|
CTGTCGACCATTTGCTTTCCTTATCTTTTGCTA
|
GTGGTGTAAGCGAAGTATTCCTCAATTTTCGACTTTCGTGCTAATTATTG
|
apfD-down-F/R
|
CAATAATTAGCACGAAAGTCGAAAATTGAGGAATACTTCGCTTACACCAC
|
ATGCGGCCGCGCTGAATGATTTAAACCGTTTGATA
|
apxIVA-F/R
|
GGCTACCCATTTCCCTTCG
|
GAGCAACAACGTCGCACA
|
qseBC-exterior-F/R
|
GTTTATCGGTAGGTCCACAGG
|
TGATCGCAGCAAATACCAAA
|
qseBC-interior-F/R
|
CGGAACGACTCGCCTCTT
|
AAATCGCCAAAATCAATAGCGGTAG
|
apfA-exterior-F/R
|
AATACCGGCTCAATTCGCTTA
|
ACCAGTCACGTAAAGGGTCGGC
|
apfA-interior-F/R
|
GGTGTCATCGTATAACCGTATT
|
TTGGCGGCATCGGCTTCTT
|
apfB-exterior-F/R
|
GTTGGATACGGATCTGTGAATAG
|
TTACGGTGGAAGGCGGAACGATTAC
|
apfB-interior-F/R
|
CGCATCATTGGTATGTAAGGTC
|
GGATTTCCGTGTTTCGAGTTT
|
apfC-exterior-F/R
|
AGGAATACTTCGCTTACACCAC
|
GTTATCACGGCTACAGAGGC
|
apfC-interior-F/R
|
CTAATCGCCCGCTTTGTT
|
CTGAATGCCGCAGTCCCT
|
apfD-exterior-F/R
|
CATCATCGGCATAACGCAGTC
|
AGGCGGATTAGTCGGAGGC
|
apfD-interior-F/R
|
GCATAAACCCAGCATACCA
|
TTCCTCTGCTTGCCTTTG
|
For complement construction
|
qseBC-F/R
|
AACTGCAGTTGCAAAATTTCAGCAAATTTAGAC
|
TTGCGGCCGCTTAGAAGATAATTTCCGCTTTTAAA
|
apfA-F/R
|
CCCTCGAGATGCAAAAACTAAGTCTTATTCGAC
|
TTGCGGCCGCTTAATTTGATGCGCAGAAATTTGCC
|
apfB-F/R
|
CCCTCGAGATGCAGTATTCCGTATGTGATGTAA
|
TTGCGGCCGCTCATACACTTTCAACATGACCTAAC
|
apfC-F/R
|
CCCTCGAGATGTTGAAAGTGTATGAATTTCATT
|
TTGCGGCCGCCTAACCGCCTCCGACTAATCCGCCT
|
apfD-F/R
|
CCCTCGAGATGAGTTCTCCGATTTTCGCATGGT
|
TTGCGGCCGCTTAGATATAAATTTTATCAATAATT
|
For RT-qPCR
|
16S rRNA-F/R
|
CCATGCCGCGTGAATGA
|
TTCCTCGCTACCGAAAGAACTT
|
apfA-qPCR-F/R
|
GACCGCCGCTACAGTTTTTA
|
TTGCCATTTTAGCTACGGTTG
|
apfB-qPCR-F/R
|
CGAAGGCGCATCATTGGTAT
|
CAAACGCAAATAAACCCGGC
|
apfC-qPCR-F/R
|
AAATTTCGCCTAATCGCCCG
|
GCCGCAGTCCCTTTAAAACA
|
apfD-qPCR-F/R
|
TGTCCCCGCTTCCCAATATT
|
TGCTTGCCTTTGGAATTGCT
|
For protein expression
|
PqseB-F/R
|
CGCCCATATGTTGCAAAATTTCAGCAAATTTAGAC
|
CGCCCTCGAGAGCATCTGAATTTCCTATTTTA
|
For EMSAs
|
apf-EMSA-F/R
|
ATCCCTCTCCTTATCATATCTAAGA
|
TTTGACCTCTGAATTTATTAAAACG
|
pilM-EMSA-F/R
|
CGAAAAATTCTCTTCTGGTAAATGA
|
AATTTTAGTTCCTTAAATCAACTTT
|
glpK-EMSA-F/R
|
CGTTTCCCTTTTTTACGGACTAAAC
|
ATAGTCCTCCTACGGATAACGAAAA
|