Fig. 4

Maximum-likelihood phylogenetic trees of the Nudiviridae, including Aratus pisonii nudivirus (ApNV). The radial plot (A) represents all available Nudiviridae DNA polymerase protein sequences and highlights the potential for two sub-families suggested in recent literature. B ApNV groups in the Gammanudivirus most closely with Homarus gammarus nudivirus (United Kingdom) and Carcinus maenas nudivirus (invasive host, Canada). Lonomia obliqua nucleopolyhedrovirus (Baculoviridae) is used as an outgroup to root the concatenated protein tree, which was developed from the following proteins: 38 k, ac81, DNApol, Helicase, lef-4, lef-5, lef-8, lef-9, p74, pif-1, pif-2, pif-3, pif-4, pif-5, pif-6, vp39 and vp91. The tree was inferred using IQ-Tree