Fig. 1From: The truncated form of flagellin (tFlic) provides the 2dCap subunit vaccine with better immunogenicity and protective effects in miceConstruction and characterization of Cap-tFlic VLPs. Amino acid sequences corresponding to tFlic were inserted into the C-terminal sequence of Cap, and recombinant proteins were specifically identified by a mouse anti-Cap mAb (8C3) using Western blotting (A). Morphological characterization of Cap VLPs by TEM (20,000 ×) (B). Determination of Cap-tFlic VLP particle diameter by DLS (C). NC, negative control, protein 60 T, ~ 60 kDa Back to article page