Fig. 2

Assessment of antigen uptake and immunostimulatory effects in BM-DCs. BM-DCs were incubated with Cap-tFlic VLPs. The primary antibody was mouse 8C3 mAb, and the secondary antibody was FITC-coupled goat anti-mouse IgG. Signals of cell nuclei (blue) and Cap (green) were detected by CLSM (A). BM-DCs were exposed to 100 μg/mL antigens for 12 h, and TNF-α, MHC-II and CD86 expression was then measured by qPCR (B). NC, unprocessed BM-DCs. **P < 0.01, ***P < 0.001, ****P < 0.0001