Fig. 1

MGF505-3R inhibited the cGAS-STING-mediated IFN-β signaling pathway. A-C HEK293T cells were cotransfected with IFN-β promoter, ISRE, or NF-κB luciferase reporter plasmids (100 ng); pRL-TK plasmid (50 ng) and the indicated plasmids (200 ng) (pCMV-N-HA was chosen as a negative control). Twenty-four hours after transfection, HEK293T cells were used for dual-luciferase reporter assays. D Twenty hours after transfection of HA-MGF505-3R, the cells were left untreated or treated with poly (dG:dC) (500 ng) for 12 h before use in dual-luciferase reporter assays. MGF505-3R expression was analyzed by western blotting using an anti-HA antibody. E-F HEK293T cells were transfected with the Flag-cGAS/Flag-STING expression plasmids (500 ng) and HA-MGF505-3R expression plasmid (1 μg). Twenty-four hours after transfection, the cells were used for qRT–PCR analysis. “+” represents transfected, “-” represents nontransfected. All experiments were repeated independently at least thrice. The data from independent experiments are presented as the mean ± SD; n=3. *P<0.05, **P<0.01, ***P<0.001