Prevalence and fluoroquinolone resistance of Campylobacter spp. isolated from beef cattle in Japan

Animal Diseases

Table 3 List of primers used in this study

Name	Sequence (5′–3′)	Target	Size (bp)	Reference
Multiplex PCR for *C. jejuni, C. fetus, C. coli, C. upsaliensis, *C. hyointestinalis, and C. lari**				Kamei et al. 2016
Cj-spBU5	ATC TTT TAA CCT TGC TTT TGC	C. jejuni	714
Cj-spBR6	GCA AGC ATT AAA ATC GCA GC
Cf-spBU6	GGC TTT GCA AAA CCA GAA G	C. fetus	553
Cf-spBR3	CAA GAG TTC CTC TTA AAC TC
Cc-spBU10	CTG TAT CAA GAC CTA GCT C	C. coli	433
Cc-spBR9	TAT AAA GCT GCA GTG TTG G
Cu-spBU5	GCC TTA GCT TTC TTT GGG	C. upsaliensis	342
Cu-spBR5	CAT CGG CTT GGA CGC GAC
ChII-spBU8	CCT AGT AGC GCT ACT TAG	C. hyointestinalis	215
ChII-spBR8	CAA ATA CCC TAC CTG TAGC
Cl-spBU4	GTA TCC ATG CTT TAT CAA GA	C. lari	141
Cl-spBR4	GTA GGC CTA TAA GAG AAC C
Amplification and sequencing of partial *gyrA* for *C. jejuni*				Zirnstein et al. 1999
GZgyrA5	ATT TTT AGC AAA GAT TCT GAT	gyrA	673
GZgyrA6	CCA TAA ATT ATT CCA CCT GT
Detection of the Thr-86-to-Ile mutation for *C. jejuni*				Zirnstein et al. 1999
CampyMAMAgryA1	TTT TTA GCA AAG ATT CTG AT	Thr-86-to-Ile mutation	265
CampyMAMAgyrA5	CAA AGC ATC ATA AAC TGC AA
Amplification and sequencing of partial *gyrA* for *C. coli*				Zirnstein et al. 2000
GZgyrACcoli3F	TAT GAG CGT TAT TAT CGG TC	gyrA	505
GZgyrACcoli4R	GTC CAT CTA CAA GCT CGT TA
Detection of the Thr-86-to-Ile mutation for *C. coli*				Zirnstein et al. 2000
GZgyrACcoli3F	TAT GAG CGT TAT TAT CGG TC	Thr-86-to-Ile mutation	192
CampyMAMAgyrA8	TAA GGC ATC GTA AAC AGC CA