Fig. 1

Results of Experiment 1. a Change in body temperature during the challenge period based on body temperature at 0 d.p.i.. b Percentage of pigs positive for virus shedding in nasal swabs collected at -2, 1, 3, and 5 d.p.v. and 0 to 5 d.p.i. c Viral titers in nasal swabs collected after vaccination and challenge. d Viral titers in BALF collected at 5 d.p.i.. (e, f) Geometric mean of serum HI antibody titers against H3N2 TX98 (e) and H3N2 CO99 (f) collected at -2, 7, 14, and 20 d.p.v. and 0 and 5 d.p.i.. (g) Percentage of each lung lobe presenting macroscopic lesions. Differences between treatment groups were determined by Tukey’s (a, g) or Dunn’s (c-f) multiple comparisons tests. Survival curves were compared using the Mantel-Cox log-rank test (b). A statistically significant difference between two groups is indicated by a star (a) or different letters (b-g). Data are represented as mean ± SEM (a-d, g) or geometric mean (e, f). Symbols represent treatment groups (a, b) or individual pigs (c-g)