Fig. 2From: MIC17A is a novel diagnostic marker for feline toxoplasmosisExpression and purification of recombinant MIC17A. A MIC17A was mainly expressed in the inclusion bodies. M: marker; 1: total lysate from culture without IPTG induction; 2: total lysate of IPTG induced culture; 3: supernatant faction of IPTG induced culture; 4: pellet faction of IPTG induced culture. B Purification of MIC17A by a nickel column. M: marker; 1: flow-through fraction after loading denatured and renatured pellets to the nickel column; 2–6: eluted fractions after washing with different concentrations of imidazole. 2: 0%, 3–4: 5%, 5–6: 10%. C Western blotting checking the reactivity of MIC17A to cat sera. M: marker; 1: incubated with Toxoplasma positive cat serum; 2: incubated with Toxoplasma negative cat serumBack to article page