Fig. 9From: Altered gene expression in human brain microvascular endothelial cells in response to the infection of influenza H1N1 virusRegulation of induced immune genes expression in human brain microvascular endothelial cells (hBMECs) following influenza A virus infection. a Canonical pathway of the “Interferon signaling” for the genes differentially expressed in hBMECs 12 hpi, genes that are significantly up-regulated are shown in red. The intensity of red corresponds to an increase in fold change levels of the cells infected with A/WSN/33 (H1N1) compared to the control cells z-score = 4.2. White nodes specify genes with no significant gene expression at 12 hpi. The pathway was generated with IPA (Ingenuity pathways system). b quantification of gene expression by (RT-qPCR) for IFN-λ 2/3 (c) ISG15, and d IFN-β. (b-d) the hBMECs were infected with A/WSN/33 (H1N1) at MOI 0.1, and the target genes were quantified by relative quantification qPCR. statistical-significance analyzed by t-test * p < 0.05, ** p < 0.01, and **** p < 0.0001. e Mechanistic network by IPA for the upstream regulators interacting with IFN-β, which enables to discover plausible sets of connected upstream regulators that can work together to elicit the gene expression changes observed in our datasetBack to article page