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Fig. 9 | Animal Diseases

Fig. 9

From: Altered gene expression in human brain microvascular endothelial cells in response to the infection of influenza H1N1 virus

Fig. 9

Regulation of induced immune genes expression in human brain microvascular endothelial cells (hBMECs) following influenza A virus infection. a Canonical pathway of the “Interferon signaling” for the genes differentially expressed in hBMECs 12 hpi, genes that are significantly up-regulated are shown in red. The intensity of red corresponds to an increase in fold change levels of the cells infected with A/WSN/33 (H1N1) compared to the control cells z-score = 4.2. White nodes specify genes with no significant gene expression at 12 hpi. The pathway was generated with IPA (Ingenuity pathways system). b quantification of gene expression by (RT-qPCR) for IFN-λ 2/3 (c) ISG15, and d IFN-β. (b-d) the hBMECs were infected with A/WSN/33 (H1N1) at MOI 0.1, and the target genes were quantified by relative quantification qPCR. statistical-significance analyzed by t-test * p < 0.05, ** p < 0.01, and **** p < 0.0001. e Mechanistic network by IPA for the upstream regulators interacting with IFN-β, which enables to discover plausible sets of connected upstream regulators that can work together to elicit the gene expression changes observed in our dataset

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