Skip to main content
Fig. 1 | Animal Diseases

Fig. 1

From: Development of a loop-mediated isothermal amplification assay for detection of Austropeplea tomentosa from environmental water samples

Fig. 1

Specificity panel used to determine AtLAMP specificity using common livestock helminths and F. hepatica.A,C LAMP amplification curves for AtLAMP performed on DNA from common livestock helminths and F. hepatica performed in duplicate (Tec - Teladorsagia circumcincta; Hc - Haemonchus contortus; Tc- Trichostrongylus colubriformis; Ta-T. axei; Ost- Ostertagia spp.; Coop- Cooperia spp.; Pcer-Paramphistomum cervi; Fh-F. hepatica; +ve-snail DNA; NTC-No template control). T. axei and Cooperia spp. recorded one of two replicates with Tp values exceeding 25 minutes, with Tp values from H. contortus, Ostertagia spp., and F. hepatica exceeding 29 minutes from one of two replicates. B,D The fluorescent derivative annealing curve (Ta) for the corresponding livestock helminths and F. hepatica DNA. No Ta values recorded for all helminth species assessed

Back to article page

Animal Diseases

ISSN: 2731-0442