Table 1 A. tomentosa LAMP primers and primer combinations assessed in this study to reduce non-target snail species amplification
Primers and snails assessed | Primer combination used and average Tp values (mm:ss) | |||
|---|---|---|---|---|
Primer set: 1 | LF + LB | LB | LF | No loops |
 Pa | 10:73 | 17:73 | 15:80 | – |
 Pc | 11:08 | 18:30 | 14:73 | – |
 Gt | 11:15 | 18:65 | 15:23 | – |
 Al | 12:00 | 19:65 | 16:23 | – |
 Av | 8:15 | 10:38 | 11:73 | 18:80 |
 At | 6:00 | 9:45 | 8:00 | 17:38 |
Primer set: 2 | LF + LB | LB | LF | No loopsa |
 Pa | 9:30 | 11:30 | 10:73 | 13:23 |
 Pc | 9:65 | 11:73 | 11:08 | 14:00 |
 Gt | 9:45 | 11:38 | 11:15 | 13:73 |
 Al | 10:30 | 12:73 | 11:15 | 14:45 |
 Av | 9:15 | 11:45 | 10:15 | 13:38 |
 At | 4:45 | 6:00 | 5:30 | 6:73 |
Primer set: 3 | LF + LB | LB | LF | No loops |
 Pa | 17:04 | 22:73 | 22:15 | Not assessed |
 Pc | 17:34 | 23:30 | 22:73 | Not assessed |
 Gt | 17:15 | 23:80 | 22:80 | Not assessed |
 Al | 18:51 | 25:50 | 24:38 | Not assessed |
 Av | 17:61 | 26:23 | 24:15 | Not assessed |
 At | 9:69 | 14:15 | 12:38 | Not assessed |