Table 4 Average time-to-positive (Tp, mm:ss) values of water samples spiked with varying quantities of A. tomentosa by LAMP
| Â | Water eDNA purification method | ||
|---|---|---|---|
DNA starting quantity (μg) | NaOAC + NaI clean up | Filtering 50 mL vol | Filtering 250 mL vol |
1 | 10:73 ± 1.03 SD | 15.15 ± 0.21 SD | 14:80 ± 0.49 SD |
0.5 | 11:80 ± 0.92 SD | 15.35 ± 0.11 SD | 14:80 ± 0.49 SD |
0.25 | 13:80 ± 2.33 SD | 16.23 ± 0.32 SD | 16:45 ± 0 SD |
0.125 | 12:80 ± 3.32 SD | 16.30 ± 0.21 SD | 17:58 ± 0.60 SD |
0 | ND | ND | ND |