Table 5 Common snails found in Australia used for DNA extraction for specificity panel. Synthetic constructs containing partial ITS-2 insert of snails and corresponding amplicon size used in this study
Species | Abbreviation | GenBank accession | pBHA vector (bp) | ITS-2 insert (bp) | Total amplicon size (bp) | Relevance |
|---|---|---|---|---|---|---|
Physa acuta | Pa | KF316328.1 | 257 | 302 | 559 | Common in South-East Australia, non-fluke transmitting |
Pseudosuccinea columella | Pc | HQ283261.1 | 535 | 792 | Common in NSW, northern VIC, fluke transmitting | |
Galba truncatula | Gt | KT280457.1 | 496 | 753 | Not in Australia, fluke transmitting | |
Austropeplea lessoni | Al | EU556308.1 | 486 | 743 | Common in Southern and Eastern Australia, non-fluke transmitting | |
A. viridis | Av | EU556313.1 | 397 | 654 | Common in central NSW, southern QLD, fluke transmitting | |
A. tomentosa | At | EU556270.1 | Â | 411 | 668 | Common in South-East Australia, key fluke transmitting species in VIC |