Development of a loop-mediated isothermal amplification assay for detection of Austropeplea tomentosa from environmental water samples

Table 6 Loop-mediated isothermal amplification primers used for Austropeplea tomentosa detection in this study

Primer name	Primer sequence 5′-3′^a	Sequence region 5′-3′
Set 1 F3	GTCTCAAGCACAAGCCGC	F3
Set 1 B3	GGGCGCCGATTTGTCAAG	B3
Set 1 FIP	GAGGAAAATTTGGCCGCCGCCCGTTGTCCGTGTTCGTC	F1,F2
Set 1 BIP	CTAACGGGCCCGCTCGTAACTCAGCGTAAGCTTCTCTCCT	B1, B2
Set 1 LF	AGAGCAAGGCGGCGT	LF
Set 1 LB	AAGCTCCAGGGTGATTGCG	LB
Set 2 F3^a	TGCACGGTGTTGCCCG	F3
Set 2 B3^a	GCGTAAGCTTCTCTCCTCCG	B3
Set 2 FIP^a	GCGGACGTCCCGAGACGAACTGGCCCCGTGGTCTCAA	F1,F2
Set 2 BIP^a	TTCCTCCTCGTCACCGCTATGCCAATCACCCTGGAGCTTGTT	B1, B2
Set 2 LF	GCGGCGGCCAAATT	LF
Set 2 LB	CGGCTCGCTCTCGCTAA	LB
Set 3 F3	GGTGGCCCCGTGGTCT	F3
Set 3 B3	CTTCTTCAATTCGTACGGGCG	B3
Set 3 FIP	AGCGGTGACGAGGAGGAAAATTCGTGTTCGTCTCGGGACGT	F1,F2
Set 3 BIP	CGTAACAAGCTCCAGGGTGATTGTTGTCAAGCGAGCGTCAGC	B1, B2
Set 3 LF	CGAGAGCAAGGCGGCGT	LF
Set 3 LB	CGGAGGAGAGAAGCTTAC	LB