Table 1 Features of different types of CRISPR systems
From: CRISPR-Cas9 mediated phage therapy as an alternative to antibiotics
Characteristic | Type I | Type II | Type III | Type IV | Type V | Type VI |
|---|---|---|---|---|---|---|
Effector complex | Multi-subunit (Class 1) | Single unit (Class 2) | Multi-subunit (Class 1) | Multi-subunit (Class 1) | Single unit (Class 2) | Single unit (Class 2) |
Signature Protein | Cas3 | Cas9 | Cas10 | Csf1 | Cas12 | Cas13 |
Target molecule | DNA | DNA | RNA/DNA | ? | DNA | RNA |
Details | Cleaves ssDNA strands | Originates blunt double strand break | Binds to RNA molecules | Most unknown CRISPR system | Original staggered- double strand break | RNA-guided RNase |