Enzootic epidemiology of Brucella in livestock in central Gansu Province after the National Brucellosis Prevention and Control Plan

Table 1 Classical biotyping assay for Brucella isolates B.melitensis TZ

Brucella spp. (Strain)	CO₂ requirement	H₂S production	Urease activity	Thionin blue^a			Basic fuchsin^b		Monospecific antisera			Brucella phage group
Brucella spp. (Strain)	CO₂ requirement	H₂S production	Urease activity	a	b	c	b	c	A	M	R	Tb	Bk₂
B.melitensis bv. 1 (M5-90)	-	-	+	+	+	+	+	+	-	+	-	-	+
B.abortus bv.1 (A19)	-	+	+	-	-	-	-	-	+	-	-	+	+
B.suis bv.1 (S2)	-	+	+	-	-	-	+	+	+	-	-	-	+
B.melitensis TZ	-	-	+	+	+	+	+	+	+	+	-	-	+

Note: + , Positive. -, Negative. ^aConcentration of Thionin blue (a = 1:25 000, b = 1:50 000, c = 1:100 000). ^bConcentration of basic fuchsin (b = 1:50 000, c = 1:100 000)