Fig. 3
From: Essential role of ATG7 in LPS-induced endometrial inflammatory injury in goats
Silencing ATG7 inhibits apoptosis in goat EECs. A Real-time PCR analysis of atg7 mRNA levels in goat EECs. B Western blot analysis of ATG7 protein levels in goat. EECs, pCD513B-U6-ATG7-shRNA-1 and mock control were transduced for 48 h. C and D Real-time PCR and Western blot analysis of the bax, bcl-2 and ATG7 levels following LPS treatment. E Representative merged fluorescence images of TUNEL in goat EECs with or without LPS treatment. EECs, endometrial epithelial cell. LPS, lipopolysaccharide. The data are presented as the means ± SD of three independent experiments. * Significant difference (p < 0.05) compared with other groups. ** Significant difference (p < 0.01) compared with other groups. *** Significant difference (p < 0.001) compared with other groups