Fig. 4From: Key roles of amylopectin synthesis and degradation enzymes in the establishment and reactivation of chronic toxoplasmosisDeletion of usp or gwd results in defective growth and formation of bradyzoites. a Intracellular replication rates of parasites under bradyzoite-inducing conditions for 36 h, as determined by the fraction of vacuoles containing 1, 2, 4, 8 and 16 or more parasites. Means ± S.D.s of four independent experiments, ****p < 0.0001, **p = 0.0074, two-way ANOVA. b Bradyzoite conversion assay. Parasites were cultured under bradyzoite-inducing conditions for 4 days before IFA staining to determine the efficiency of bradyzoite transition. Means ± S.D.s of four independent experiments, **p = 0.0074, ****p < 0.0001, Student’s t testBack to article page