Fig. 5

Deletion of usp or gwd affects virulence and cyst formation in vivo. a Survival curves of ICR mice infected with 100 tachyzoites of the indicated strains (n = 10 mice or more), *p = 0.0218, **p = 0.0041, Gehan–Breslow–Wilcoxon test). b Toxoplasma cysts in the brains of ICR mice that survived at day 30 in a. The brain cysts were collected and stained with DBA-FITC, and then counted. Median with interquartile range, ***p = 0.0006, ****p < 0.0001, Student’s t-test. c Relative size (diameter) of brain cysts detected in Fig. 4b, *p = 0.029, ****p < 0.0001, Student’ s t test