Fig. 1

Anti-PRV effect of eleven Chinese herbal extracts. Monolayer cells were incubated with CHE and the PRV HNX strain (MOI 0.01) for 1 h and washed three times with PBS. Infected cells were then incubated in medium with or without extracts for 23 h. The antiviral activity of extracts against PRV in Vero cells was detected by plaque reduction assay (A) and photographed by microscopy (B). The nucleocapsid (DAPI, blue) and the gE protein of PRV (gE, green). The viral DNA fragments in the cell culture supernatant were tested by qPCR (C). All data are represented as the mean ± SD, (n = 3), *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, vs the positive control