Fig. 3

The inhibitory effect of five potential antiviral extracts on PRRSV infection. Vero cells were infected with WUH3 PRRSV (MOI = 0.01) and incubated either with or without extracts. (A) At 23 h postinfection, the infection range of PRRSV in cells was observed by microscopy. The nucleocapsid (DAPI, blue) and the PRRSV protein (green). (B) The intracellular total RNA fragments obtained from the attached cells were measured by RT‒qPCR. (C) The PRRSV RNA fragments in the supernatant were tested by qPCR. All data are represented as the mean ± SD, (n = 3), *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, vs the positive control