Fig. 4

Analysis of cellular apoptosis during T. gondii infection. a Schematic of transwell chamber analysis. Chicken erythrocytes were placed in the upper chamber, and T. gondii RH tachyzoites, secretions, and lysates were placed separately in the lower chamber. b Cellular apoptosis was analyzed by flow cytometry. After incubation of chicken erythrocytes with tachyzoites, secretions, and lysates for 24 h, the proportion of live cells was evaluated using flow cytometry. Flow cytometry showed necrotic cells at the upper left and normal cells at the lower left. Late apoptotic cells are shown on the upper right; late apoptotic cells are shown on the lower right. Experiment without dead cells was used as Control 1, experiment without apoptotic cells was used as Control 2, experiment with only living cells was used as Control 3, experiment without living cells was used as Control 4. The relative fluorescence intensity is represented by an axis