Reference intervals for hematology, plasma biochemistry, and bone mineral density in captive Ceratophrys cranwelli (Anura: Ceratophryidae)

Long, Siyu; Qiao, Yujing; Zhou, Siyu; Muhammad-Farooq, Tareen; Shen, Yaoqin

doi:10.1186/s44149-023-00099-3

Table 1 Descriptive statistics and significant analysis in erythrocytes and nuclei

From: Reference intervals for hematology, plasma biochemistry, and bone mineral density in captive Ceratophrys cranwelli (Anura: Ceratophryidae)

Variable	n	Mean ± SD	Median	Min	Max	RI (95% CI)	P value	P value (sex)
L	500	18.36 ± 1.19	18.31	14.29	23.11	18.32–18.41	< 0.001	0.371
♂	250	18.38 ± 1.20	18.37	14.29	23.11	18.30–18.45	0.100
♀	250	18.36 ± 1.18	18.28	14.64	22.99	18.31–18.41	< 0.001
W	500	11.09 ± 0.81	11.06	8.00	13.00	11.06–11.11	0.010	0.095
♂	250	11.05 ± 0.78	11.01	8.50	12.97	11.00–11.10	0.200
♀	250	11.11 ± 0.82	11.08	8.01	12.99	11.07–11.14	0.020
L/W	500	1.66 ± 0.15	1.65	1.21	2.40	1.66–1.67	< 0.001	0.096
♂	250	1.67 ± 0.16	1.66	1.28	2.27	1.66–1.68	0.005
♀	250	1.66 ± 0.15	1.65	1.21	2.40	1.65–1.67	< 0.001
NL	500	5.85 ± 0.56	5.81	4.33	7.75	5.80–5.90	0.004	0.991
♂	250	5.86 ± 0.57	5.84	4.49	7.75	5.79–5.93	0.004
♀	250	5.84 ± 0.56	5.80	4.33	7.53	5.77–5.91	0.358
NW	500	4.02 ± 0.37	4.02	3.07	5.27	3.99–4.05	0.074	0.088
♂	250	4.02 ± 0.36	4.02	3.19	5.27	3.98–4.06	0.053
♀	250	4.02 ± 0.38	4.03	3.07	5.07	3.97–4.06	0.334
NL/NW	500	1.47 ± 0.21	1.44	1.01	2.36	1.45–1.49	< 0.001	0.677
♂	250	1.471 ± 0.21	1.44	1.04	2.31	1.44–1.50	< 0.001
♀	250	1.469 ± 0.21	1.45	1.01	2.36	1.45–1.50	0.001
S	500	159.58 ± 17.22	157.93	117.35	220.36	158.06–161.09	< 0.001	0.740
♂	250	160.13 ± 16.9	157.40	117.35	220.36	158.02–162.24	< 0.001
♀	250	159.01 ± 17.55	158.43	118.67	210.32	156.83–161.20	0.330
NS	500	18.45 ± 2.33	18.37	13.13	26.32	18.25–18.66	0.001	0.982
♂	250	18.48 ± 2.25	18.37	13.56	25.27	18.20–18.76	0.002
♀	250	18.42 ± 2.42	18.39	13.13	26.32	18.12–18.72	0.103
NS/S	500	0.117 ± 0.016	0.116	0.07	0.17	0.115–0.118	0.404	0.637
♂	250	0.117 ± 0.017	0.116	0.07	0.16	0.115–0.119	0.509
♀	250	0.116 ± 0.015	0.116	0.07	0.17	0.114–0.118	0.924

Length and width in μm and size in μm²
Abbreviations: CI Confidence interval, L Erythrocyte length, W Erythrocyte width, L/W Length/width, NL Nucleus length, NW Nucleus width, S Erythrocyte size, Max Maximum, Min Minimum, NS Nucleus size, NS/S Nucleocytoplasmic ratio, n Cell number, SD Standard deviation, RI Reference interval

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