Fig. 3

Reactivities of mAbs to multiple virus strains. A HI titer of ascites of mice inoculated with antibody-positive hybridoma cell lines. B SDS‒PAGE analysis of purified mAbs. C Western blot detection of mAb reactivity with H9N2-W1 virus. Inactivated H9N2-W1 viruses were subjected to SDS‒PAGE, and western blotting was performed using the mAbs as the primary antibody. D Immunofluorescence assay detection of mAb reactivity with H9N2-W1 and H9N2-115 viruses. MDCK cells infected with H9N2-W1 or H9N2-115 were incubated with the mAbs as the primary antibody and FITC-conjugated goat anti-mouse IgG as the secondary antibody. Nuclei were stained with DAPI. Scale bars = 20 μm